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huprot human proteome microarray v3.0 (CDI Laboratories)

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CDI Laboratories huprot human proteome microarray v3.0
Huprot Human Proteome Microarray V3.0, supplied by CDI Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huprot+human+proteome+microarray+v3%2E0/huprot+human+proteome+microarray/pmc10614366-160-16-21
Average 90 stars, based on 1 article reviews

huprot human proteome microarray v3.0 - by Bioz Stars, 2026-09

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Article Title: Discovery of potential biomarkers for lung cancer classification based on human proteome microarrays using Stochastic Gradient Boosting approach.
Article Snippet: Purpose Early identification of lung cancer (LC) will considerably facilitate the intervention and prevention of LC.. The human proteome micro-arrays approach can be used as a “liquid biopsy” to diagnose LC to complement conventional diagnosis, which needs advanced bioinformatics methods such as feature selection (FS) and refined machine learning models.. Methods A two-stage FS methodology by infusing Pearson’s Correlation (PC) with a univariate filter (SBF) or recursive feature elimination (RFE) was used to reduce the redundancy of the original dataset.

Article Title: WASF2 Overexpression and Promoter Hypomethylation Are Associated With Poor Clinical Outcomes in Hepatocellular Carcinoma
Article Snippet: 22K human protein microarray Protein microarray experiments were performed using a HuProt human proteome microarray v3.0 (CDI Laboratories Inc., Mayaguez, PR, USA) with 20 human plasma samples [ ve patients with liver cirrhosis, ve patients with HCC (at diagnosis, 6 months before diagnosis, and 1 year before diagnosis)].

Article Title: Circulating small extracellular vesicle-derived splicing factor 3b subunit 4 as a non-invasive diagnostic biomarker of early hepatocellular carcinoma
Article Snippet: Autoantibodies that were highly expressed in HCC were identified by performing protein microarray experiments using a HuProt human proteome microarray v3.0 (CDI Laboratories Inc., Mayaguez, PR, USA).

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Identification of HDAC6 as a novel NEDD8-binding partner (A) Schematic illustration of strategy for profiling the NEDD8-binding proteins using a protein microarray (left panel). Total 17 proteins containing NEDD8 were identified as a NEDD8 binding proteins (right panel). (B) HeLa cells were transfected with mCherry-NEDD8 and GFP-HDAC6, and the colocalization of two proteins was analyzed in the absence or presence of MG132 (5 μM). Enlarge insets represent the NEDD8/HDAC6-colocalized structure observed in perinuclear region upon MG132 treatment. Nuclei were stained with DAPI (blue). DMSO was used as a negative control. (C) Recombinant GST and GST-HDAC6 were loaded in 8%–16% SDS-PAGE and visualized by staining with Coomassie Brilliant Blue (left panel). Dot blot assay was performed with recombinant NEDD8 and HDAC6 as indicated. NEDD8-binding activity to HDAC6 was detected by anti-NEDD8 antibody (right panel). GST was used as a negative control. (D) The kinetic interaction of NEDD8 to HDAC6 was monitored at pH 4.0 using the Octet QKe System (ForteBio) as describe in Transparent Methods. See also <xref ref-type=

Figure S1 . " width="100%" height="100%">

Journal: iScience

Article Title: Stress-induced NEDDylation promotes cytosolic protein aggregation through HDAC6 in a p62-dependent manner

doi: 10.1016/j.isci.2021.102146

Figure Lengend Snippet: Identification of HDAC6 as a novel NEDD8-binding partner (A) Schematic illustration of strategy for profiling the NEDD8-binding proteins using a protein microarray (left panel). Total 17 proteins containing NEDD8 were identified as a NEDD8 binding proteins (right panel). (B) HeLa cells were transfected with mCherry-NEDD8 and GFP-HDAC6, and the colocalization of two proteins was analyzed in the absence or presence of MG132 (5 μM). Enlarge insets represent the NEDD8/HDAC6-colocalized structure observed in perinuclear region upon MG132 treatment. Nuclei were stained with DAPI (blue). DMSO was used as a negative control. (C) Recombinant GST and GST-HDAC6 were loaded in 8%–16% SDS-PAGE and visualized by staining with Coomassie Brilliant Blue (left panel). Dot blot assay was performed with recombinant NEDD8 and HDAC6 as indicated. NEDD8-binding activity to HDAC6 was detected by anti-NEDD8 antibody (right panel). GST was used as a negative control. (D) The kinetic interaction of NEDD8 to HDAC6 was monitored at pH 4.0 using the Octet QKe System (ForteBio) as describe in Transparent Methods. See also Figure S1 .

Article Snippet: To address this, we utilized the HuProt v3.0 human proteome microarray (CDI Laboratories, USA), which contain over 21,000 human proteins, to identify proteins that bind directly to NEDD8.

Techniques: Binding Assay, Microarray, Transfection, Staining, Negative Control, Recombinant, SDS Page, Dot Blot, Activity Assay